The Spectacular Spider-Man

Peter Parker / Spider Man (voice) (26 episodes)

Gwen Stacy (voice) (24 episodes)

Flash Thompson (voice) (20 episodes) / Flash Thompson / Car Thief #1 (voice) (1 episode)

Sally Avril (voice) (7 episodes) / Betty Brant (voice) (3 episodes) / Sally Avril / Betty Brant (voice) (2 episodes) / Voice of OsCorp (voice) (1 episode) / Stephanie Briggs (voice) (1 episode) / Sally Avril / Computer Voice (voice) (1 episode) / Sally Avril / Coffee Clerk (voice) (1 episode) / Sally Avril / Erin (voice) (1 episode) / Sally Avril / Voice of OsCorp (voice) (1 episode) / Sally Avril / Bette Brant (voice) (1 episode)

Liz Allan (voice) (17 episodes) / Liz Allan / Helena (voice) (1 episode) / Liz Allan / Trina (voice) (1 episode)

Mary Jane Watson (voice) (16 episodes) / Mary Jane Watson / Rosie Thompson (voice) (1 episode) / Mary Jane Watson / Hermia (voice) (1 episode)

Harry Osborn (voice) (11 episodes) / Harry Osborn / Frederick Foswell (voice) (4 episodes) / Patch / Homunculi (voice) (1 episode) / Harry Osborn / Alan O'Neil (voice) (1 episode) / Harry Osborn / Patch / Frederick Foswell (voice) (1 episode)

May Parker (voice) (16 episodes)

George Stacy (voice) (9 episodes) / Rhino / George Stacy (voice) (3 episodes) / Alex O'Hirn (voice) (2 episodes) / Alex O'Hirn / Ox (voice) (1 episode) / Alex O'Hirn / Rhino / George Stacy (voice) (1 episode)

Norman Osborn (voice) (15 episodes)

Rand Robertson (voice) (6 episodes) / Rand Robertson / Robbie Robertson (voice) (4 episodes) / Robbie Robertson (voice) (1 episode) / Fancy Dan / Ricochet / Rand Robertson (voice) (1 episode) / Rand Robertson / Fancy Dan (voice) (1 episode) / Fancy Dan / Homunculus #1 (voice) (1 episode)