Your Friendly Neighborhood Spider-Man

May Parker (voice) (10 episodes) / Roxanna Volkov (voice) (2 episodes) / Mrs. Eberhardt (voice) (1 episode)

Harry Osborn (voice) (10 episodes) / Mr. Campbell (voice) (1 episode)

Peter Parker / Spider-Man (voice) (10 episodes)

Norman Osborn (voice) (10 episodes)

Lonnie Lincoln (voice) (10 episodes)

Nico Minoru (voice) (10 episodes)

Pearl Pangan (voice) (10 episodes)

Asha (voice) (4 episodes) / Mrs. Lincoln (voice) (2 episodes) / Emma (voice) (2 episodes) / Emma / Mrs. Lincoln (voice) (1 episode)

Coach Grayfield (voice) (3 episodes) / Dimitri Smerdyakov (voice) (1 episode) / James 'Speed Demon' Sanders (voice) (1 episode) / Dmitri Smerdyakov (voice) (1 episode) / Dmitri Smerdyakov / Chameleon (voice) (1 episode)

Big Donovan (voice) (6 episodes) / Big Donavan (voice) (1 episode)

Dr. Connors (voice) (3 episodes) / Dr. Connors (voice) (1 episode) / Hot Rod (voice) (1 episode) / Susan O'Harra (voice) (1 episode)